Journal: Stem cell reports
Article Title: Viral delivery of L1CAM promotes axonal extensions by embryonic cerebral grafts in mouse brain.
doi: 10.1016/j.stemcr.2023.02.012
Figure Lengend Snippet: Figure 2. Mouse embryonic cerebral cortex contains subcerebral projecting neurons and proteins that respond to each axon guidance molecule (A) Schematic of the brain slices of the E14.5 mouse frontal cortex. (B) Immunofluorescence images for CTIP2 (green), L1CAM (red), DCC (red), NRP1 (red), NRP2 (red), and DAPI (blue). Lower panels are magnified images. Arrows indicate dual positive cells for CTIP2 and L1CAM, DCC, NRP1, and NRP2, respectively. Scale bars, 20 mm (upper) and 10 mm (lower).
Article Snippet: The primary antibodies used were rat anti-L1CAM antibody (1:500; #MAB5674; R&D Systems, Minneapolis, MN), mouse anti-L1CAM antibody (1:500; #ab24345; Abcam, Cambridge, UK), rabbit anti-NRP1 antibody (1:500; #AB10521; EMD Millipore Corporation, Temecula, CA), rabbit anti-NRP2 antibody (1:500; #AB10522; EMDMillipore Corporation), goat anti-DCC antibody (1:1,000; #sc-6535; Santa Cruz Biotechnology, Heidelberg Germany), rat anti-CTIP2 antibody (1:1,000; #ab18465; Abcam), rabbit anti-GFP antibody (1:1,000; #598; Medical and Biological Laboratories, Nagoya, Japan), mouse anti-FLAG M2 antibody (1:1,000; #F1804; SigmaAldrich), rabbit anti-KI67 antibody (1:500; #ab16667; Abcam), and rabbit anti-mCherry antibody (1:500; #ab167453; Abcam).
Techniques: